Authors:

Ankita Prasad

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About Paper:

The development of elastin-like polypeptide-based nanoparticles has been shown to have potential in various therapeutic applications, specifically in the delivery of drugs to cancer cells as a mechanism of treatment. This research aims to utilize an ELP (Elastin-Like Polypeptide) fused with a polycation component and mRNA to formulate a self-assembling nanoparticle and test its efficacy for bladder cancer treatment. The ELP, isolated from a bacterial pellet by organic solvent extraction, is attached to the CDPLR has a positive charge, is complexed with negatively charged mRNA, which therefore promotes the self-assemblage of spherical nanoparticles. The mRNA encodes for the functional protein that is being delivered to cells. Initial characterization techniques involved determining the size of the nanoparticles using DLS (dynamic light scattering), determining stability by zeta potential, and using a luminescence reporter to test its performance in T24 human bladder cancer cells. The results of characterization indicate the formation of nanoparticles with different sizes upon varying amounts of polycation. An encapsulation efficiency test indicated successful entrapment of mRNA within the self-assembly. Testing the nanoparticles in cancer cells also showed the successful transfection of mRNA and the ability to deliver the gene of interest. This research aims to develop individualized treatment of bladder cancer using self-assembling nanoparticles that rely on an ELP-based delivery system. Further testing can help indicate the viability of the nanoparticles in a clinical setting. Keywords: Elastin-Like Polypeptide; Drug Delivery; Nanoparticles; Bladder Cancer

Source:

Purdue University / 2024

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Co-authors:

Ankita Prasad