Authors:

Shreya Sinha

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About Paper:

Cells are constantly subject to various levels of mechanical stimulation in the body which can alter their cytoskeleton and govern the expression or suppression of genes involved in maintaining the extracellular matrix. Synoviocytes are connective tissue cells found in the inner lining of joint cavities that are responsible for production of synovial fluid components to ensure healthy and smooth joint movement. Excessive strain on these cells can lead to injurious states and contribute to conditions like osteoarthritis (OA), a degenerative joint disease. Therefore, it is important to understand what changes are occurring to affect components of the synovial fluid to eventually make strides in mitigating the harmful consequences of this disease. To address this, a cell stretching device that was previously developed depends on the magnitude and frequency of air pressure applied to the polydimethylsiloxane membrane on which the SW982 cell line is cultured. Using this device, sinusoidal mechanical stimulation at 1 Hz frequency will be applied uniaxially, equibiaxially, and non- equibiaxially at magnitudes between 5% to 15% under a live cell imaging microscope to measure morphological changes. Cell layer will be tested using quantitative reverse transcription polymerase chain reaction for gene expression, and surrounding growth media will be tested with a hyaluronan enzyme-linked immunosorbent assay to measure hyaluronan concentration. Preliminary results have shown an overall transformation in shape and branching of cells after 1 hour of stretching. Future work can specifically analyze signaling pathways of the studied genes to find areas to target for OA relief and prevention. Keywords: Synoviocyte; Cell Stretch; Gene Expression; Extracellular Matrix; Osteoarthritis

Source:

Purdue University / 2024

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No topics listed

Co-authors:

Shreya Sinha