Authors:

Rubyleane Linton

Date Created:

Not specified

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About Paper:

Cache Valley Virus (CVV) is a rare but dangerous orthobunyavirus that causes abortions and congenital disabilities in small ruminants, such as goats and sheep. The virus is zoonotic, having implications for human health as well. Mosquitoes are considered the primary vector of CVV. It is an emerging pathogen in the U.S and recent serologic testing of small ruminants suggests this virus is present in Indiana. This study aimed to identify CVV in Indiana by testing mammal-biting mosquito species collected from different parts of the state. A previously published real-time reverse transcriptase PCR was verified for use in the lab and utilized to detect target sequences of CVV within nucleic acids extracted from mosquito pools collected in 2023-2024. The mosquito species that were analyzed include Aedes albopictus, Aedes canadensis, Aedes j.japonicus, Aedes vexans, Anopheles punctipennis, Anopheles quadrimaculatus, Coquillettidia perturbans, and Culex pipiens (mixed). While the study is ongoing, no CVV was detected in the 45/324 pools of mosquitoes tested to date. Detection of positive pools would confirm the presence of CVV in the state. Additionally, testing various species of mosquitoes from different parts of the state would provide information regarding the location of the virus and the species of mosquitoes responsible. Traps that collect these mosquitoes mimic preferred hosts and environments to attract particular species. Infected mosquito species knowledge is essential in determining which traps will be the most effective in Indiana to increase the possibility of detecting this virus in the mosquito vector. Keywords: Cache; Valley; Virus; Mosquitoes; Indiana

Source:

Purdue University / 2024

Topics:

No topics listed

Co-authors:

Rubyleane Linton