Dylan
Wade Seets
Investigating the application of Reference Point Indentation as a possible tool to determine quality metrics for excised keel bones. Life Sciences
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Authors:
Dylan Wade Seets
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About Paper:
Assessing keel bone damage (KBD) is a subjective process based upon visual observation or manual palpation. There are no standard bone quality assessment techniques as keels are remarkably fragile. Reference point indentation (RPI) is a minimally invasive method for assessing bone quality properties via microindentations, which has never been applied to keels. We wanted to develop a protocol for collecting RPI data on keels utilizing the BioDent RPI system. Visible KBD was noted then keels were trimmed of cartilage, measured, then sectioned into thirds and labeled: Tip, Middle, End. Keels were secured and indented from End to Tip. We are presenting Indentation Distance Increase (IDI) measures since IDI has been cited as an indicator of bone resistance. Statistical analyses included an ANOVA (P < 0.05) comparing the IDI between the 3 keel sections; a Tukey test was used for mean separation. Average IDI values in the Tip were numerically higher compared to Middle and End sections indicating more fragile bone tissue. Within 4-8 mm of a tip fracture, a higher IDI value than other Tip measures occurred. IDI decreased within the Middle with the most variability between bones and most KBD noted. One keel was measured twice: IDI was twice as large on the repeated analysis in the Middle (P = 0.030; t-test) but not different in others. Variance leads the RPI system to be used as a destructive test for keels. Further experimentation should focus on KBD to better grasp bone quality impact and assessing immature keel bones. Keywords: Reference Point Indentation; Bone; Keel; Keel Bone Damage; Laying Hens
Source:
Purdue University / 2024
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Co-authors:
Dylan Wade Seets