Isabelle
Agarwal
The Impact of GDF11 on Astrocytes and Microglia
Abstract profile. Full document pending author claim.
Authors:
Isabelle Agarwal, Kaylee Wells, Lee Rubin
Date Created:
2025-01-01
Course Title:
Professor:
Not specified
About Paper:
Aging is characterized by widespread cellular damage and chronic exploring GDF11’s impacts on both cell types in vivo and in vitro. low-grade inflammation, functioning as the primary risk factor for several neurodegenerative diseases. Within the central To analyze GDF11’s role in vivo, immunofluorescence staining nervous system, inflammation can induce both neurotoxic and was performed with anti-GFAP (astrocytes), anti-MBP (myelin), and anti-IBA1 (microglia) antibodies on 3-month-old GDF11 KO neuroprotective reactive states. One suchstate, neurotoxic reactivemice and control mice, analyzing the cortex, corpus callosum, astrogliosis, plays a significant role in Alzheimer’s Disease, and hippocampus. To investigate GDF11’s impact on astrocytes Multiple Sclerosis, Parkinson’s Disease, and more. directly,astrocytesisolatedfromearlypostnatalmicewereisolated To combat aging and its effects, Growth Differentiation Factor andtreatedinvitrowithrecombinantGDF11orRepSox,aTGFβRI 11 (GDF11), a member of the transforming growth factor beta inhibitor, under both reactive and non-reactive conditions. (TGFβ) family, is under study as a potential therapeutic. The Rubin Lab previously found cognitive deficits and decreased Preliminary results suggest a sex-specific increase in microglial myelin levels in inducible GDF11 knockout (KO) mice, indicating number in male GDF11 KO mice, indicating that GDF11 may differentially regulate neuroinflammation depending on sex. GDF11 impacts the aging process through glial cells. Bulk Should our hypothesis be correct, this study would confirm that RNA sequencing of hippocampal tissue from GDF11 KO mice GDF11 plays a role in the induction of reactive astrogliosis, revealed downregulation of GFAP, a known marker of astrocytes and reactive astrogliosis, suggesting that GDF11 may contribute potentially acting through microglia to do so. Depending on to astrocyte function and reactivity. Since microglia can initiate whether this reactivity is neuroprotective or neurotoxic, GDF11 could either decrease or increase age-related neurodegeneration, astrocytic reactivity upstream through inflammatory cytokine influencing both morbidity and mortality. signaling, this project focuses on both microglia and astrocytes,
Abstract:
Aging is characterized by widespread cellular damage and chronic exploring GDF11’s impacts on both cell types in vivo and in vitro. low-grade inflammation, functioning as the primary risk factor for several neurodegenerative diseases. Within the central To analyze GDF11’s role in vivo, immunofluorescence staining nervous system, inflammation can induce both neurotoxic and was performed with anti-GFAP (astrocytes), anti-MBP (myelin), and anti-IBA1 (microglia) antibodies on 3-month-old GDF11 KO neuroprotective reactive states. One suchstate, neurotoxic reactivemice and control mice, analyzing the cortex, corpus callosum, astrogliosis, plays a significant role in Alzheimer’s Disease, and hippocampus. To investigate GDF11’s impact on astrocytes Multiple Sclerosis, Parkinson’s Disease, and more. directly,astrocytesisolatedfromearlypostnatalmicewereisolated To combat aging and its effects, Growth Differentiation Factor andtreatedinvitrowithrecombinantGDF11orRepSox,aTGFβRI 11 (GDF11), a member of the transforming growth factor beta inhibitor, under both reactive and non-reactive conditions. (TGFβ) family, is under study as a potential therapeutic. The Rubin Lab previously found cognitive deficits and decreased Preliminary results suggest a sex-specific increase in microglial myelin levels in inducible GDF11 knockout (KO) mice, indicating number in male GDF11 KO mice, indicating that GDF11 may differentially regulate neuroinflammation depending on sex. GDF11 impacts the aging process through glial cells. Bulk Should our hypothesis be correct, this study would confirm that RNA sequencing of hippocampal tissue from GDF11 KO mice GDF11 plays a role in the induction of reactive astrogliosis, revealed downregulation of GFAP, a known marker of astrocytes and reactive astrogliosis, suggesting that GDF11 may contribute potentially acting through microglia to do so. Depending on to astrocyte function and reactivity. Since microglia can initiate whether this reactivity is neuroprotective or neurotoxic, GDF11 could either decrease or increase age-related neurodegeneration, astrocytic reactivity upstream through inflammatory cytokine influencing both morbidity and mortality. signaling, this project focuses on both microglia and astrocytes,
Source:
Harvard / Harvard College | Cabot House | Neuroscience | 2027 / 2025
Topics:
gdf11, astrocyte, microglia, impact, reactive, mice, aging, factor, disease, role, neurotoxic, anti
