Authors:

Elizabeth Agyei

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About Paper:

DMP8/9, membrane-associated proteins only found on the surface of plant sperm cells, were recently discovered to aid in gamete fusion in A. thaliana. DMP8/9 are part of a bigger protein complex organized by TET11/12 tetraspanins. The knockout of dmp8/9 results in improper fertilization of the egg and central cells by the sperm cells. Overexpression of DMP9 in vegetative tissues results in reduced plant growth and problems with osmoregulation. We believe this phenotype is caused by the deregulation of ion transports or localization of membrane-associated ion transporters. The first part of this project aims to investigate the biological function of DMP9 by examining how the overexpression and knockout lines respond to ABA, H2O2, organic acids, and toxic cations. The second part aims to gain more insight into stomata response to ABA, acetic acid, and various concentrations of H2O2 in the overexpression line using epidermal peels. This project aims to present a possible model of how the overexpression of DMP9 may be interfering with plant osmoregulation and further hypothesize how this directly correlates to the function of DMP8/9 on the surface of plant sperm cells. Keywords: Osmoregulation; Double Fertilization; Sperm-Expressed Protein; Gametes; Stomata

Source:

Purdue University / 2024

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Co-authors:

Elizabeth Agyei