Authors:

Anasofia Carrillo

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About Paper:

Meristems and stem cells play essential and conserved roles in driving plant growth and development and determining biomass production and yields. We have been using two model species-the flowering plant Arabidopsis and the fern Ceratopteris-to characterize regulators in the control of meristem development and stem cell homeostasis. Among them, we identified and named two close homologs (the CrPER1 and CrPER2 genes). We investigated the CRISPR-Cas9 mediated mutations of CrPER1 and CrPER2 in transgenic Ceratopteris. Specifically, we designed two DNA primers, the forward primer 267-1 and reverse primer 267-2, to amplify the genomic region of each gene in individually transformed Ceratopteris plants. Following DNA extraction, PCR, and DNA purification, we analyzed Sanger sequencing results of a total of 88 samples to determine putative mutations in their respective target sequences. To date, we identified 3 heterozygous mutations in the target sequences of CrPER1 but have not yet identified any detectable editing for CrPER2. The potential morphological changes of these mutants will be examined in subsequent generations. The comparative evaluation with A. thaliana is ongoing to dissect the potentially conserved or divergent capabilities of these genes between ferns and angiosperms. This research demonstrates the capacity of the CRISPR technology in fern genetic research and suggests different efficiency for each individual target gene. It provides a foundation for further studies of meristem development in ferns, potentially offering insights into the evolution of plant developmental mechanisms. Keywords: [no keywords provided]

Source:

Purdue University / 2024

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Co-authors:

Anasofia Carrillo