Authors:

Samantha Lares

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About Paper:

Cold stress is a major contributor to production loss in tropical origin crop species like tomato (solanum lycopersicum). Much is known about the cold response of temperate origin plants such as Arabidopsis thaliana. However, less is known about the regulation of the cold response in tropical origin crop species, such as tomatoes, which are sensitive to above zero but near freezing temperatures. The tomato cold stress response results in the gene expression which is mediated by the master transcription factors. To further investigate the transcription factors regulating the tomato cold stress response, three knockout lines with mutations in the RAV2, C2H2, and HSF transcription factor families were grown to investigate the transcriptional response under chilling stress (4°C). These transcription factor families were chosen using 1000+ publicly available RNAseq data to identify novel transcription factors that were the most influential in potentially regulating gene expression of known cold stress related genes. When exposed to chilling stress, the tomato undergoes damage to membrane integrity, production of reactive oxidative stress and photosynthesis deficiency. We have investigated these cold stress phenotypes in three different mutant lines using plant wilting assay, electrolyte leakage, DAB staining, and measuring chlorophyll content. Analysis of these phenotypes will provide an understanding of the role our novel transcription factors play in the tomato cold stress response. We anticipate that mutation of transcription factors will alter the overall tomato cold tolerance. Keywords: Cold Stress; Gene Regulation; Tomato

Source:

Purdue University / 2024

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Co-authors:

Samantha Lares