Authors:

Adrian Muñoz

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About Paper:

Sulfur deficiency is an emerging problem in soybean (Glycine max) due to less atmospheric Sulfur deposition caused by stricter environmental regulations. Sulfur is an essential macronutrient for soybeans in part because it is required for symbiotic nodulation with nitrogen fixing Rhizobia bacteria. GmLBD5 is a class II Lateral Organ Boundary Domain (LBD) transcription factor, many members of which participate in abiotic stress responses. Preliminary results show that LBD5 is responsive to abiotic stresses and is highly expressed in nodules. To evaluate GmLBD5's role in Sulfur deficiency, we grew soybeans in a hydroponic system under both normal and reduced Sulfur conditions. The expression of GmLBD5 and its duplicate paralog, GmLBD45, was measured in roots and nodule tissues under both Sulfur treatment levels. GmLBD5 overexpression and CRISPR/Cas9 knockout constructs targeting GmLBD5 and GmLBD45 were transformed from the soybean cultivar Williams 82. This study will deepen our understanding on how the GmLBD gene family responds to abiotic stresses, paving the way to future functional studies. Keywords: Soybeans; Sulfur Deficiency; LBD genes; Abiotic Stress; Symbiotic Nodulation

Source:

Purdue University / 2024

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Co-authors:

Adrian Muñoz