Authors:

Sadie A Poirier

Date Created:

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About Paper:

The silver chloride (AgCl) reference electrode is widely employed in electrochemical and biological investigations due to its well-defined potential, cost-effectiveness, and lower toxicity when compared to mercury-containing electrodes such as the saturated calomel electrode. The commercially available AgCl reference electrode features silver wire in Cl- solution, with a porous frit at the base allowing ion exchange between sample solution into the reference electrode. However, this frit presents two substantial challenges: it limits the potential for miniaturization to the nanoscale, and it allows the leakage of silver and chloride ions into the sample solution. Previous attempts have been made to address these issues while retaining the benefits of the AgCl reference electrode, such as the bipolar reference electrode (BPRE). The BPRE replaces the frit with a sealed conductive wire, minimising leakage and allowing for miniaturization. Although theoretically leakless, these BPREs have been shown to leak small quantities of silver ions into sample solution. This is an especially pressing issue at the nanoscale, particularly since silver is toxic to biological systems and can significantly impact studies involving cells or other biological materials. In this study, we examined the impacts of continuous exposure of triple-negative breast cancer cells (TNBC) to commercially available reference electrodes and BPREs from 0 to 6 hours. Flow cytometry was used to assess cell viability after this incubation period. Keywords: Electrochemistry; Cellular Biology; Toxicology

Source:

Purdue University / 2024

Topics:

No topics listed

Co-authors:

Sadie A Poirier