Authors:

Jamie Kim Bryan

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About Paper:

RNA helicases play vital roles in regulating gene expression and transcription processes. Our present study explores the roles of the DEAD-box helicase, Dbp2, in budding yeast Saccharomyces cerevisiae and its human ortholog, DDX5. While Dbp2 is known to be involved in RNA processing, its specific functions in transcription termination require further investigation. Through high-throughput transient transcriptome sequencing (TT-seq), it was found that Dbp2 plays a crucial role in transcription termination. In this study, through a series of termination reporter assays, we validate our findings that Dbp2 plays a pivotal role in transcription termination, with DBP2 knockouts resulting in read-through defects. Additionally, mammalian DEAD-box helicase DDX5 is associated with the proliferation of small-cell lung cancer (SCLC). Several studies show that DDX5 itself undergoes multiple post-translational modifications, which contribute to its involvement in the development of different types of cancer. In our current study, our lab identified unique phosphorylation sites in DDX5 that can be attributed to its role in SCLC progression. Through site-directed mutagenesis studies (SDM), we will take a closer look into the roles of these critical phosphorylation sites in SCLC proliferation. Our findings will provide insights into the roles of DEAD-box helicases in modulating gene expression. Furthermore, it has the potential to reveal vital clues for the treatment of SCLC. Keywords: RNA Helicase; DBP2; DDX5; Transcription; Small-Cell Lung Cancer

Source:

Purdue University / 2024

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No topics listed

Co-authors:

Jamie Kim Bryan