Authors:

Molly Elizabeth Kosiba

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About Paper:

MILDEW RESISTANCE LOCUS-O (MLO) proteins are a plant specific gene family involved in a variety of functions such as powdery mildew susceptibility, reproduction, and root tropism. Within the model species Arabidopsis, there are 15 MLO proteins which were recently discovered to function as calcium channels. Calcium signaling is an important mechanism within plants as it aids in the response to abiotic and biotic stress and helps in the development of tip-growing cells, such as root hairs and pollen tubes. Although there are 15 MLO proteins in Arabidopsis, many have yet to be characterized. In order to better understand the role of MLO proteins in plant development, previously generated MLO overexpressor and knockout lines were phenotyped. The phenotypes observed were primary root and root hair length, rosette area, and flowering time. The results of the phenotyping were compared to the previous findings in Gao et al., 2022 on whether these proteins could function as calcium channels. The subcellular localization of these proteins was then compared to the phenotyping results to understand if subcellular localization affects the protein's ability to function as a calcium channel. In addition, for the overexpressor lines that displayed an interesting phenotype, the plants were GUS stained to determine whether the native promoter is active in the tissues where the phenotype was observed. Through the study of MLO overexpressor or knockout lines and the resulting plant's phenotype, we can better understand how each MLO protein contributes to plant development. Keywords: Plant Biology; Development; Arabidopsis

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Purdue University / 2024

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Co-authors:

Molly Elizabeth Kosiba