Authors:

Erica Ann Long

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About Paper:

Prevotellaceae is a gram-negative bacterial family composed of the genera Prevotella, Alloprevotella, Hallella, and Paraprevotella that has been found in the pig gut. Although a positive correlation between Prevotellaceae and growth performance in pigs has been reported, our understanding of the biology and function of Prevotellaceae and its impact on pig production remains limited. In the current study, we hypothesized that as a known dietary fiber fermenter, Prevotellaceae will show an increased prevalence within the gut microbiome of pigs that have been fed a high-fiber diet. Fecal samples were taken from grower pigs (body weight around 31 to 32 kg) being fed a control diet or a high-fiber diet (n = 4) that contains dehydrated alfalfa meal and beet pulp. Fecal DNA was extracted for 16S rRNA gene amplicon sequencing on an Illumina MiSeq platform. Prevotellaceae tended to be increased by the high-fiber diet compared with the control group (mean value, 10.05% vs. 5.56%), which was largely attributed to an enrichment of ASV14 (Alloprevotella) and ASV39 (Prevotellaceae NK3B31 group). The genomic capacity of Prevotellaceae species that have been reported in the pig gut was further investigated based on publicly available genome sequences. The species composition of Prevotellaceae varied among individuals. Analyses based on the ten identified Prevotellaceae species from pig feces demonstrated that Prevotellaceae members may fill a unique niche in the gut and contributes to metabolic functionality. Future work to identify and characterize Prevotellaceae at strain-level will be critical to understand its function and biological properties in pigs. Keywords: Prevotellaceae; Pig; Gut Microbiome; Fiber

Source:

Purdue University / 2024

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Co-authors:

Erica Ann Long