Authors:

Grace Anne Stanton

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About Paper:

Many studies have been conducted on the carnivorous plant genus-Nepenthes; however, most of these studies focus on describing the growth stages and morphology of the pitchers. There is a gap in knowledge within this field of study on the tropism of the plant and its effects the placement of the pitchers. There is no known tropism-the movement of an organism in response to external stimuli-for Nepenthes sp. This project was focused on assessing the plant's molecular response to external stimuli; this response could be quantified through following the signal transduction pathway. A calcium ion was chosen as an indicator of pathways activation due to its pervasiveness through many different pathways. Calcium selective microelectrodes were fabricated using glass micropipettes, an electrolyte, calcium selective LIX solution, and silver wire. These microelectrodes were used in conjunction with SIET hardware and ASET-LV4 software to map the fluctuation of calcium ions within various sections of the plant. This map shows which sections of the plant have a reaction to the external stimuli and the intensity of the response. The larger concentration of calcium ions is indicative of a greater signaling response. Mapping signaling pathways through the quantification of calcium ions will shine a light on the tropism of Nepenthes sp. Keywords: Calcium; Nepenthes; ASET-LV4; SIET; Molecular Signaling

Source:

Purdue University / 2024

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Co-authors:

Grace Anne Stanton