Authors:

Abigail J Dressman

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With its central role in digestion and nutrient absorption, the intestinal epithelium is arguably one of the most critical components of the gastrointestinal system. Studying this specialized tissue in the native context of living organs, however, presents significant challenges due to its anatomical complexity and cellular heterogeneity. This necessitates the development of physiologically relevant, context-specific experimental models for research purposes. To address this need, we introduce intestinal organoids as a model system that emulates an anatomical unit of the intestine responsible for essential functions such as nutrient absorption and maintaining homeostasis. Specifically, our system uses living intestinal stem cells grown in a three-dimensional environment to recreate the intricate architecture of the intestinal epithelium, including the villi and crypt structures. Using intestinal organoids, we demonstrate in vitro reconstruction of this intestinal epithelium that mimics the stages of intestinal development from the formation of the gut tube to the patterning of crypt-villus regions. We demonstrate the utility of this model system by i) measuring organoid growth and ii) analyzing budding morphologies in intestinal organoids. Keywords: Organoids; Intestinal Epithelium; Morphogenesis

Source:

Purdue University / 2024

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Co-authors:

Abigail J Dressman