Authors:

Gayatri Pradeep

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About Paper:

Chronic Kidney Disease (CKD) describes the gradual deterioration of the structure and function of the kidney. As renal function declines, the risk of bone fracture escalates, increasing morbidity and mortality rates. The current gold standard for fracture risk evaluation is dual-energy X-ray absorptiometry; however, its efficacy is questionable as fractures occur despite CKD patients displaying healthy bone mineral densities (BMD). This highlights the importance of utilizing alternative biomarkers such as collagen and water to predict fracture risk. This study utilized 80 samples of rat tibiae (40 normal and 40 Cy/+ with n=8 harvested at 22, 25, 28, 31, and 34 weeks) to run through thermogravimetric analysis (TgA). The weight loss curve was analyzed with each peak representing loss of free water, bound water, organic proteins, and structural water respectively. 0.35 mm cross sectional slices of the distal end of the tibiae were obtained to undergo analysis using Fourier Transform Near-Infrared Spectral Imaging (FT-NIRSI). Each cross-sectional slice was heated to 60 °C (free water peak) and 110 °C (loosely bound water peak) and spectral images were taken to observe changes in bone water localization. Additionally, a PTH ELISA assay was run on cardiac serum to measure parathyroid hormone (PTH) levels. Results obtained from the TgA indicated that Cy/+ rats displayed significantly higher levels of free water at 22 and 24 weeks, lower levels of loosely bound water at 25 and 34 weeks, and lower levels of collagen water at 31 weeks. The analysis of FT-NIRSI and PTH ELISA results are underway. Keywords: Bone Imaging; Chronic Kidney Disease

Source:

Purdue University / 2024

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Co-authors:

Gayatri Pradeep