Authors:

Maitreyee Panini Telang

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About Paper:

Bioelectricity refers to the endogenous electrical signals generated and mediated by the movement of ions across the cell membrane. Bioelectricity plays crucial physiological roles in various biological systems and processes, such as embryonic development, regeneration, and cancer. Researchers have been using chemogenetic tools, DREADDs (Designer Receptors Activated by Designer Drugs), and GEVI (Genetically Encoded Voltage Indicators), to manipulate and visualize in-vivo bioelectric activities in primate and murine models. Zebrafish have extensively been used for studying embryonic development and modeling diseases due to their advantages in tractable genetics and early embryo transparency. However, these tools are not available in zebrafish. To visualize and manipulate in vivo bioelectricity, this study aims to apply and validate the function of DREADD and GEVI in the zebrafish model. We have generated a series of transgenic zebrafish lines. Each fish line contains a DREADD and a GEVI component under the UAS promoter, and the transgene insertion has been validated by PCR. This study aims to validate the GEVI reporter of the transgenic fish lines by crossing UAS-DREADD-GEVI fish lines with tissue-specific Gal4 fish lines. Our experiment shows that GEVIs are visible in some fish lines and indicates the tools can be employed to control membrane potential in zebrafish. We expect these zebrafish lines will serve as a valuable resource for research in bioelectricity across fields like neuroscience, cardiology, and developmental biology. Keywords: Bioelectricity; DREADD; GEVI; Zebrafish

Source:

Purdue University / 2024

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Co-authors:

Maitreyee Panini Telang