Authors:

Maggie Kim

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About Paper:

One of the defining pathological hallmarks of Alzheimer's disease (AD) is tau aggregation. It has been reported that these aggregates form fibers with two prominent morphologies: straight filaments and paired helical filaments. In addition, tau aggregates from post-mortem AD patients have been found to contain metal ions, suggesting that the dysregulation of metal homeostasis is linked to tau aggregation by propagating said process. Though the influence of metals on tau aggregation has been studied before, there has not been many reports of the cryo-EM structure of tau aggregates with any metals prevalent to AD. Using ThT fluorescence we monitor the kinetics of tau aggregation in the presence of different metals and further validate if there are any observable morphological differences through AFM and TEM. From our initial TEM results, we notice the fibers are reminiscent of paired helical filaments in the presence of Zn2+ and further investigate it using Cryo- EM. Keywords: Alzheimer; Tau; AFM; TEM; Cryo-EM

Source:

Purdue University / 2024

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Co-authors:

Maggie Kim