Authors:

Hurshal Pol

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About Paper:

Per- and polyfluoroalkyl substances (PFAS) are a group of persistent organic pollutants contaminating drinking water and food sources. Perfluorooctanesulfonic acid (PFOS), the most prevalent PFAS chemical, accumulates in the brain resulting in motor and behavior disorders such as Parkinson's Disease and ADHD. Previous studies show neurotransmitters such as dopamine, serotonin, and glutamate are impacted by PFOS exposure, but little is known about the relationship between PFOS accumulation in the brain and the spatial distribution of neurotransmitters. We hypothesize that PFOS-specific accumulation in the brains of exposed rats will correlate with changes in neurotransmitter levels in the same regions. To test this, we will utilize Desorption Electrospray Ionizing-Mass Spectroscopy Imaging (DESI-MSI) to visualize and quantify PFOS accumulation and neurotransmitter levels in brain sections. Brain tissue from Sprague-Dawley rats dosed with 10 mg/kg PFOS or vehicle via oral gavage will be collected for DESI-MSI analysis at timepoints 1-90 days post-exposure. In a pilot experiment, we imaged coronal rat brain sections to discern the distribution of neurotransmitters and determined endogenous neurotransmitters were not detectable without chemical derivatization. Methods are being developed for future tissue treatment. Additionally, droplet tests with various concentrations of PFOS in water were performed to determine the limit of detection to ensure biologically relevant concentrations could be imaged. The detectable range included 200mg/L-67.5µg/L PFOS. This study characterizes the distribution of both PFOS and neurotransmitter distributions in the brain to elucidate mechanisms of neurotoxicity. Understanding these mechanisms can pave the path for counteracting the adverse outcomes of PFOS exposure. Keywords: PFOS; Neurotoxicity; DESI-MSI; Neurotransmitters; Ppersistent Organic Pollutant

Source:

Purdue University / 2024

Topics:

No topics listed

Co-authors:

Hurshal Pol