Authors:

Abigail Martin

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About Paper:

Bioelectricity, which is characterized by electrical signaling across cell membranes through the distribution of charged molecules, has been thoroughly investigated in neuronal and muscular systems. Recently, its involvement in embryonic development has been recognized. Determining the role of bioelectricity in embryonic development can help decipher the regulatory mechanisms for developmental patterning, regeneration, and cancers. Many genetically encoded voltage indicators, or GEVIs, have been reported in many publications to study the bioelectricity in neuronal systems of several model organisms. However, it is not known which GEVIs are suitable for studying zebrafish embryonic development, especially in a tissue- specific manner. To first test which GEVIs work in zebrafish, we cloned a few recently developed GEVIs and made Tol2 constructs under the UAS promoter. By injecting the constructs into neuronal promoter-driven Gal4 fish lines, it was determined whether the signals could be visualized. Secondly, to achieve the tissue-specific membrane voltage signals, corresponding promoter-driven Gal4 fish lines were characterized. Finally, to understand tissue-specific bioelectricity during embryonic development, membrane voltage was visualized in different tissues using corresponding promoters. Upon finishing our experiments, functional GEVIs in zebrafish can be determined. Tissue-specific bioelectricity can be informative to reveal the roles of bioelectricity during embryonic development. Keywords: Bioelectricity; Zebrafish; GEVI

Source:

Purdue University / 2024

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Co-authors:

Abigail Martin