Parnitha
Bandla
88 Program for Research in Science and Engineering Neuron Quantification of Acetone-Impacted Axolotl Brains
Abstract profile. Full document pending author claim.
Authors:
Parnitha Bandla, Celeste Wu, Jessica Whited
Date Created:
2025-01-01
Course Title:
Professor:
Not specified
About Paper:
Elucidating the regenerative process in axolotls (Ambystoma cement was applied to close the wound. The heads were harvested, mexicanum), a model organism for whole-limb regeneration, placedinfour timepointsfor acetoneimmersion: 72, 24, 12, anda may have therapeutic potential for limb amputees. Although controlof0hours,andneuronsizeanddensityfromeachgroupwas regeneration is now understood to be a body-wide process, the quantified with beta-III tubulin staining using ImageJ. The control relationship between the brain and regeneration is unknown. established a neuron diameter of 22:151▯m, with the 24 hour This project conducts preliminary tests supporting a larger-scale condition displaying slight, insignificant shrinking at 18:596▯m. experiment measuring neural electrical signals via an implanted Immunofluorescenceimagesfromthe72hourtimepointdisplayed probe. Implantation surgery and probe retrieval from the brain widespread tissue degradation with a neuron size of 19:927▯m depend on the application and eventual removal of dental cement From the control’s neuron size, a neuron density of 30 neurons per whichisdissolvedwithacetone. However,acetoneishypothesized electronicprobewascalculated. Thisfundamentalinformationwill to shrink axolotl neurons, affecting neuron density which relates optimizefutureprobeimplantationsurgeriesandsignalrecordings, to the number of neurons recorded by each probe. Acetone leadingtoamorestreamlinedprocessofimplanting,recording,and also damages morphological and structural integrity. In this analyzing electrical signals via probe. experiment, four axolotls underwent shortened brain surgery, and
Abstract:
Elucidating the regenerative process in axolotls (Ambystoma cement was applied to close the wound. The heads were harvested, mexicanum), a model organism for whole-limb regeneration, placedinfour timepointsfor acetoneimmersion: 72, 24, 12, anda may have therapeutic potential for limb amputees. Although controlof0hours,andneuronsizeanddensityfromeachgroupwas regeneration is now understood to be a body-wide process, the quantified with beta-III tubulin staining using ImageJ. The control relationship between the brain and regeneration is unknown. established a neuron diameter of 22:151▯m, with the 24 hour This project conducts preliminary tests supporting a larger-scale condition displaying slight, insignificant shrinking at 18:596▯m. experiment measuring neural electrical signals via an implanted Immunofluorescenceimagesfromthe72hourtimepointdisplayed probe. Implantation surgery and probe retrieval from the brain widespread tissue degradation with a neuron size of 19:927▯m depend on the application and eventual removal of dental cement From the control’s neuron size, a neuron density of 30 neurons per whichisdissolvedwithacetone. However,acetoneishypothesized electronicprobewascalculated. Thisfundamentalinformationwill to shrink axolotl neurons, affecting neuron density which relates optimizefutureprobeimplantationsurgeriesandsignalrecordings, to the number of neurons recorded by each probe. Acetone leadingtoamorestreamlinedprocessofimplanting,recording,and also damages morphological and structural integrity. In this analyzing electrical signals via probe. experiment, four axolotls underwent shortened brain surgery, and
Source:
Harvard / Ezekiel Bajomo, Grace Atteh, Benjamin Freedman / 2025
Topics:
neuron, axolotl, brain, probe, regeneration, acetone, regenerative, proces, cement, control, experiment, electrical
