Authors:

Kashyap Akkinapally

Date Created:

Not specified

Course Title:

Professor:

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About Paper:

Bioelectric signaling during embryonic development remains largely uncharacterized, although recent work suggests that bioelectric signals play a crucial role in this process. Here, we examine a mutant zebrafish Dhi4458, in which the inwardly rectifying potassium channel gene, kcnj10a, causes elongation of all the fins. We hypothesize that the ectopic expression of kcnj10a during somitogenesis causes bioelectric changes in the fish mutant compared to wildtype fish. To test this hypothesis, we utilize a transgenic reporter fish line, Tg(ubi: ASAP1), which encodes Accelerated Sensor of Action Potential 1, to measure membrane potential in 18- to 24-hour-old fish embryos. At the 6-8 somite and 15-somite stage, I did not find a significant difference in membrane potential changes using a fluorescent microscope. So far, no conclusion can be drawn due to the data quality and limited sample size. I will utilize a confocal microscope to enhance sensitivity, examine more fish embryos across various developmental stages, and optimize analysis methods in the future. Keywords: Bioelectricity; Developmental Biology; Somitogenesis; Embryonic Development; Zebrafish

Source:

Purdue University / 2025

Topics:

No topics listed

Co-authors:

Kashyap Akkinapally