Authors:

Zhiyuan Chen

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About Paper:

Chromosomal disorders are genetic mutations that affect individual health on a cellular level throughout the body. Chromosomal 8p is a critical region that's crucial for physical growth and cognitive development. However, de novo 8p mutations which cause cancer, developmental delays, and malformations have been underexplored despite their clinical importance. Patient-derived human-induced pluripotent stem cells (hiPSCs) are valuable tools in drug and mutation screenings due to their ability to differentiate into various cell types, enabling the modeling of disease-relevant tissues and testing drug efficacy in vitro. Commercial media kits can provide insight into the process of differentiating iPSCs into neurons and cardiomyocytes, which can later help optimize small molecule-driven differentiation, offering more economical and batch-consistent results. This project aims to develop and optimize protocols for ventricular cardiomyocyte differentiation of 8p patient cell lines. Neurogenesis and ventricular cardiomyocyte generation of 8p patient-derived hiPSCs were tested for downstream studies. Cell viability, differentiation efficacy, and cell morphology were evaluated among various differentiation protocols for the cell lines tested. The differentiation timeline, predicted mechanism, and cell response were compiled for various protocols and target cell types. Fluorescent microscopy and cell painting were conducted for in- depth screening of morphological differences. Results from cell painting provide quantitative data on cellular morphology and subcellular organization, setting a phenotype baseline for differentiation protocols on a molecular level. Future work may focus on mutation screening and drug testing for ventricular cardiomyocytes with unique types of 8p mutations. Keywords: Stem Cell; Chromosome; Mutations; Differentiation; Cardiomyocyte

Source:

Purdue University / 2025

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No topics listed

Co-authors:

Zhiyuan Chen