Authors:

Francisco Sebastiano

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About Paper:

Electric pulses of sufficient duration and intensity can permeabilize cells through electroporation or induce cell death through apoptosis. This study will investigate the biological response to electric pulses below this threshold at high repetition rates, on the order of kilohertz, and unlikely to noticeably heat immortalized human leukemia cells. Cell viability and mitochondrial function are measured using trypan blue exclusion and tetrazolium salt (MTT) proliferation, respectively, 24 and 48 hours after exposure. Experimental results for the minimum repetition rate and number of pulses necessary to induce bioeffects are compared to simulated membrane pore formation. These results will demonstrate the relationship between pulse repetition parameters and bioeffect thresholds for trains of short duration, low energy electric pulses. Keywords: Electroporation; Apoptosis; Pulsed Power

Source:

Purdue University / 2025

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No topics listed

Co-authors:

Francisco Sebastiano