Authors:

Wilhelm S Smith

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About Paper:

Doxorubicin, a powerful anthracycline chemotherapy drug, has been linked to cardiac dysfunction in 50% of patients post-cancer treatment. Cardiac strain is a sensitive metric for detecting dysfunction, but limitations in its measurement with 2D speckle-tracking echocardiography prevent robust regional assessment. Here, we aim to characterize regional left ventricular strain in a murine model of acute doxorubicin-induced cardiotoxicity using a novel 4D image analysis MATLAB program. Seven male C57Bl/6J mice were injected intraperitoneally with doxorubicin (DOX; 25 mg/kg body weight dosage); 3 control mice were injected with equal volumes of saline. Echocardiography was performed for all mice at baseline before DOX injection and 2 days after; control mice and 3 DOX mice were also imaged at 7 days. To assess cardiac performance under exertion, a dobutamine stress test was performed for all non-baseline timepoints, reacquiring select images after dobutamine injection. 4D ultrasound images were obtained using the Vevo 3100 and analyzed using a MATLAB program developed by the Cardiovascular Imaging Research Laboratory at Purdue. Metrics of interest include regional and global longitudinal and circumferential strain; ejection fraction, left ventricular mass, and E/A wave ratio were also measured. This research hopes to further characterize the acute changes in cardiac strain induced by DOX; future work includes histological assessment and mass spectrometry imaging of cardiac tissue to further characterize injury and a more clinically relevant chronic investigation of cardiotoxicity in mice. Keywords: Doxorubicin; 4DUS; Ejection Fraction; Echocardiography

Source:

Purdue University / 2025

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Co-authors:

Wilhelm S Smith