Authors:

Haley Jane Harmeson

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About Paper:

Glaucoma is one of the leading causes of irreversible blindness in both humans and canines. Increased intraocular pressure (IOP) and its negative impact, such as apoptosis and degeneration on retinal ganglion cells (RGCs) and the optic nerve, is a primary risk factor for glaucoma. In comparison to dogs with other forms of glaucoma, clinical observations suggest that Beagle dogs that display ADAMTS10 mutations may exhibit more favorable visual outcomes despite elevated IOP. However, it remains unclear whether this clinical impression reflects an intrinsic effect of the ADAMTS10 mutation, the gradual elevation of IOP typically seen in this form of glaucoma, or other unknown factors. From previous studies, our group developed a canine ocular hypertensive glaucoma model with controlled IOP elevations, allowing consistent application of the same magnitude and duration of IOP elevation. In this model, the target IOP range of 36-45 mmHg can best resemble the human glaucomatous disease mechanism. Using this preliminary model, we induced controlled ocular hypertension to compare its effects on glaucoma progression between ADAMTS10-mutant (n=1) and non- mutant (n=1) dogs. The progression of glaucoma was evaluated based on optical coherence tomography (OCT), electroretinography (ERG), fundus photography (Retcam), maze vision testing, and histopathology. Preliminary in vivo results include (1) ADAMTS10-mutant dog showed dramatic fundus photography changes including altered tapetal reflectivity during IOP elevation. (2) The ADAMTS10-mutant dog showed decreased ERG values and decreased visual performance in maze vision testing. (3) In OCT, subjectively, the ADAMTS10-mutant dog appeared to have more retinal thinning. Unlike the clinical impression, when the same magnitude and duration of IOP elevation was induced, ADAMTS10-mutant dog might be more susceptible to IOP-induced optic neuropathy. Keywords: Glaucoma; Optic; Neuropathy † Presenting Undergrad Author; ‡ Contributing Undergrad Author; * Undergrad Acknowledgment

Source:

Purdue University / 2025

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Co-authors:

Haley Jane Harmeson