Authors:

Maria Margineantu

Date Created:

Not specified

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About Paper:

Lysosomal membrane proteins are critical for various cellular signaling processes and digestion. The Ragulator complex, which includes LAMTOR1 and LAMTOR2 proteins, is essential for mTORC1 activation. This pathway is involved in cell growth and cell metabolism. Post- translational modifications (PTMs) of such proteins may have a significant influence for assembly of the complex and their function. Isolating and examining the LAMTOR1 and LAMTOR2 subunits can lead towards better understanding of their roles in signaling and PTMs. Immunoprecipitation using antibodies specific to both LAMTOR1 and LAMTOR2 were performed using cultured mammalian cells. Western blot analysis then confirmed successful enrichment of both the LAMTOR proteins. These findings support further investigation on how PTMs can regulate lysosomal signaling and may provide insights on their roles in disease related functions. Keywords: [no keywords provided]

Source:

Purdue University / 2025

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No topics listed

Co-authors:

Maria Margineantu